IL-1 beta ELISA Kit

Catalog No. ABIN5520913

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-1 beta. There are 2 publications available.

Quick Overview for IL-1 beta ELISA Kit (ABIN5520913)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.906 pg/mL - 250 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details IL-1 beta ELISA Kit

Minimum Detection Limit: 3.906 pg/mL

Purpose: For quantitative detection of IL-1β in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of IL-1β. No significant cross-reactivity or interference between IL-1β and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between IL-1β and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 2.344 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

References for IL-1 beta ELISA Kit (ABIN5520913)

Jonas, Magoń, Waligóra, Seweryn, Podolec, Kopeć: "High‑density lipoprotein cholesterol levels and pulmonary artery vasoreactivity in patients with idiopathic pulmonary arterial hypertension." in: Polish archives of internal medicine, Vol. 128, Issue 7-8, pp. 440-446, (2018) (PubMed).

Kim, Kim, Kim: "The effects of a single bout pilates exercise on mRNA expression of bone metabolic cytokines in osteopenia women." in: Journal of exercise nutrition & biochemistry, Vol. 18, Issue 1, pp. 69-78, (2015) (PubMed).

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL-1beta

Background: IL-1β/IL-1beta/IL1B/IL1-BETA/IL1F2/catabolin

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome